Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell Instruction Manual(western)

Note
To insure the best performance from the Trans-Blot SD semi-dry electrophoretic transfercell, become fully acquainted with these operating instructions before using the cell to transfer samples. Bio-Rad recommends that you first read these instructions carefully. Then assemble and disassemble the cell completely without transferring sample. After these preliminary steps, you should be ready to transfer a sample.
Bio-Rad also recommends that all Trans-Blot SD cell components and accessories be cleaned with a suitable laboratory cleaner (such as Bio-Rad Cleaning Concentrate, catalog number 161-0722) and rinsed thoroughly with distilled water, before use.
Warranty
Bio-Rad Laboratories warrants the Trans-Blot SD semi-dry electrophoretic transfer cell
against defects in materials and workmanship for 1 year. If any defects occur in the instrument during this warranty period, Bio-Rad Laboratories will repair or replace the defective parts free.The following defects, however, are specifically excluded:
1. Defects caused by improper operation.
2. Repair or modification done by anyone other than Bio-Rad Laboratories or an authorized agent.
3. Use of fittings or other spare parts supplied by anyone other than Bio-Rad Laboratories.
4. Damage caused by accident or misuse.
5. Damage caused by disaster.
6. Corrosion due to use of improper solvent or sample.
This warranty does not apply to parts listed below:
1. Platinum plate electrode.
For any inquiry or request for repair service, contact Bio-Rad Laboratories after confirming the model and serial number of your instrument.
Section 1
Introduction
Blotting was first performed by Southern in 1975 with the transfer of DNA from agarose gels to nitrocellulose membranes. Blotting has subsequently been applied to RNA and protein from both agarose and polyacrylamide gels. Membrane materials have been expanded to include PVDF for improved protein binding capacity. To overcome the inefficiency of capillary transfers, electric current has been adopted for eluting proteins from polyacrylamide gels, as first described by Towbin et al. in 1979. Since that time, electrophoretic transfer has also been used for DNA and RNA blotting.
For blotting PCR fragments, plasmid and vector DNA, and RNA with the SD cell, use theTrans-Blot SD DNA blotting kit. DNA or RNA can be blotted from agarose gel to Zeta-Probe GT membrane in only 10 minutes, without any gel pretreatments. The kit comes complete with DNA/RNA blotting accessories and a detailed instruction manual.
Semi-dry blotting was first reported by Kyhse-Andersen in 1984. Blotting was performed with plate electrodes in a horizontal configuration. The gel and nitrocellulose membrane were sandwiched between sheets of buffer-soaked filter paper, which served as the ion reservoir and replaced the buffer tank. The plate electrodes, separated only by the filter paper stack, provided high field strength (V/cm) across the gel, and very efficient, rapid transfers.
The Trans-Blot semi-dry transfer cell incorporates the original concepts of semi-dry blotting along with innovative features for quick set-up and ease of use. The platinum-coated titanium and stainless steel electrode pair provides efficient, background-free blotting with trouble-free service.
Section 3
Safety Instructions
Read the entire manual before beginning electrophoretic transfers.
Electrophoretic transfer of proteins and nucleic acids is dependent on many factors. Observe the following guidelines to avoid mishaps that may result in serious damage to the instrument or injury to the operator.
1. Do not reverse polarity on this instrument.
This will result in corrosion and rusting of the stainless steel cathode. If this should occur, the stainless steel should be cleaned with a mild abrasive cleaner to remove the rust.
2. Do not exceed 25 V with this instrument.
This could damage the electrodes.
3. Do not adjust the pH of transfer buffers unless specifically indicated.
Follow instructions carefully. Adjustment of pH of transfer buffers, when not indicated, will result in increased buffer conductivity. This is manifested by a higher than expected initial current output as shown by the power supply's current meter. Monitor buffer resistance with the Model 200/2.0 power supply prior to each run to insure proper buffer conductivity.
4. Lengthy transfer times are not recommended.
Do not leave this instrument unattended. Joule heat can be generated rapidly during semi-dry blotting. Transferring longer than 2 hours can damage the unit.
5. Power supply requirements.
The Trans-Blot SD cell should only be used with the microprocessor-controlled Model 200/2.0 power supply (catalog numbers 165-4761 and165-4762), or the Model 1000/500 power supply (catalog numbers 165-4710 and 165-4711). Do not use the Model 250/2.5 power supply with this apparatus. The low voltage, high current operating conditions of the Trans-Blot SD cell are not compatible with the Model 250/2.5 power supply, and will cause the power supply to blow a fuse.
6. Do not operate this instrument in ambient temperatures exceeding 50 °C.
Important
This Bio-Rad instrument is designed and certified to meet IEC 1010-1* safety standards. Certified products are safe to use when operated in accordance with the instructtion manual. This instrument should not be modified in any way. Alteration of this instrument will:
• Void the manufacturer's warranty
• Void the IEC1010-1 safety certification
• Create a potential safety hazard
Bio-Rad is not responsible for any injury or damage caused by the use of this instrument for purposes other than for which it is intended or by modifications of the instrument not performed by Bio-Rad or an authorized agent.
*IEC 1010-1 is an internationally accepted electical safety standard for laboratory instruments.

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